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    RPMI 1640 Medium
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      RPMI 1640 Medium

      货号:AYJ0095A-100g
      包装:100g
      • 品牌:NISSUI

      *西宝提示:我司所销售的化学试剂、原料等所有产品(包括但不限于抗生素类、蛋白质类、试剂盒类产品等)仅限用于科学研究用途,不得作用于人体。

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      Directions
      Dissolve 10.2 g of the medium in distilled water and adjust the volume to 1,000mL. Sterilize by autoclaving at 121°C for 15 minutes. Cool the sterilized medium to room temperature and add a proper amount of sterile 10% sodium bicarbonate. (pH will be 7.1-7.4 at 37°C, in an atmosphere of 5% CO2 in air by adding 18-30 mL of sodium bicarbonate solution.) Aseptically add 0.3 g Lglutamine and the desire amount or serum.

      Summary
      RPMI 1640 is a medium for suspension culture investigated by George E. Moore, former director of Roswell Park Memorial Institute. Up to now, such media were not autoclavable because of decomposition or deterioration of the constituents, so that sterilization had been performed by filtration using a membrane filter. RPMI 1640 Medium “Nissui” 2 is an autoclavablepowdered medium specially manufactured to overcome the difficulties associated with filtersterilized media, such as contamination by Mycoplasma. This medium has growth promoting effect to primary cultures of mouse and human leukemia cells, various kinds of cell lines and KATO-III cells.
      Storage and Expiration
      Close tightly and store at 2-5°C in a dark and dry place. May be stored up to one (1) year.
      Precautions
      Do not use the product after its expiry date.
      Quality of the product is not warranted after its shelf life.
      When if medium or reagent touched eyes or mouth, immediately wash with plenty of water, and consult a physician. Any medium, reagent and materials must be sterilized by autoclaving or boiling water after use, and then disposed as industrial waste according to the law or regulations related to dispose.

      Formula

      Components
      In 10.2 g/L
      L-Arginine Hydrochloride
      240.0 mg
      L-Asparagine, H2O
      56.8 mg
      L-Asparatic Acid
      20.0 mg
      L-Cysteine Hydrochloride, H2O
      72.9 mg
      L-Glutamic Acid
      20.0 mg
      Succinic Acid
      46.0 mg
      Sodium Succinate,6 H2O
      164.0 mg
      Glutathione
      1.0 mg
      Glycine
      10.0 mg
      L-Histidine Hydrochloride, H2O
      20.3 mg
      L-Hydroxyproline
      20.0 mg
      L-Isoleucine
      50.0 mg
      L-Leucine
      50.0 mg
      L-Lysine Hydrochloride
      40.0 mg
      L-Methionine
      15.0 mg
      L-Phenylalanine
      15.0 mg
      L-Proline
      20.0 mg
      L-Serine
      30.0 mg
      L-Threonine
      20.0 mg
      L-Tryptophan
      5.0 mg
      L-Tyrosine
      20.0 mg
      L-Valine
      20.0 mg
      Biotin
      0.2 mg
      Calcium Pantothenate
      0.25 mg
      Choline Chloride
      3.0 mg
      Folic Acid
      1.0 mg
      Inositol
      35.0 mg
      Nicotinamide
      1.0 mg
      p-Aminobenzoic Acid
      1.0 mg
      Pyridoxine Hydrochloride
      1.0 mg
      Riboflavin
      0.2 mg
      Thiamin Hydrochloride
      1.0 mg
      Cyanocobalamin
      0.005 mg
      Sodium Chloride
      6,000.0 mg
      Potassium Chloride
      400.0 mg
      Calcium Nitrate
      69.5 mg
      Monosodium Phosphate
      677.0 mg
      Magnesium Sulfate
      48.8 mg
      Glucose
      2,000.0 mg
      Phenol Red
      5.0 mg

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