Gel chromatography
Gel chromatography separates bimolecular on the basis of size. This technique uses a molecular sieve in the form of polysaccharide gel that comprises spherical beads. As these beads are tiny and porous, so they allow only smallmoleculesto enter the gel, while the largermoleculesremain outside these beads. Therefore, when the column is eluted with an appropriate buffer, largermoleculespassout through the column. At a faster rate then smaller ones. This technique is also called as size exclusive chromatography.

Phases of chromatography
The components to be separated are distributed between two phases: astationaryphase and a mobile phase, which percolates through astationarybed. Thestationaryphase basically comprises a column field with porous beads.
Column packing
Spherical porous beads of defined sizes are used to pack the chromatography column. The porous gel may comprise cross-linked dextrans (sephadex), cross-linked poly-acrylamide (bio- gel P) or cross-linked agarose (sepharose). When columns are created they are packed with porous beads with a specific pore size so that they are mostaccurateat separatingmoleculeswith similar to the pore size.

Method
Asamplecomprising a mixture ofproteinsis dissolved in the same solvent that is running through the column. The variousmoleculesof this mixture get flushed through the column at different rates. The separation of thesemoleculesdepends on whether they can fit with in the pore size of packing material.

As a molecule flow through the column, itpassesby a no. of porous beads. Smallmoleculesenters the porous beads and hence, their rate of passage through the gel is retarded as compared to that of the larger molecule that can not fit into the pores and continue moving along the solvent. So, molecule with the larger size reaches the end of the column before the molecule with smaller size do. Small amount of solvent eluted through the gel are collected in differenttubesand theproteinpresent in eachsampleare analyzed.

Applications of the gel chromatography
Gel chromatography is a very efficient method for the separation, analyzing and characterization of the mixture of macro-molecules.
1. In nay chemical or bioprocessing industry, separation and purification of a product from complex mixture is very important. Gel chromatography can separate complex mixtures with great precision.
2. Chromatography is well suited to a variety of uses inthe fieldof biotechnology, such as separating mixture ofproteins.

Related products:

彩票手机投注平台（837709.OC），2003 年成立于张江高科技园区，现已成为生命科学领域集研发、生产、贸易、进出口、技术服务一体化的综合服务商。女足世界杯开户主营包括IVD原料，科研试剂，新材料和健康产品四大板块和服务。除自营Seebio和Canmedo(康美多)、泉养堂、五藏养生品牌外，女足世界杯开户代理了Wako、APSC、Jackson、Ludger、Bioporto、Lumiprobe、Aalto等知名品牌。女足世界杯开户正致力于分子诊断、转化医学、干细胞、抗衰老等研发领域，依托产学研创新平台，打造大健康产业链，以持续不竭的技术创新、稳定可靠的优质产品和专业便捷的技术服务，提供更具价值、创新性和定制化的综合解决方案，与您携手共创美好的明天。

官网：www.cxbio.com	微信服务号：iseebio	微博：seebiobiotech

商城：mall.cxbio.com	微信订阅号：seebiotech	泉养堂：www.canmedo.com